PREVALENCE AND RESISTANCE AGAINST DIFFERENT ANTIMICROBIAL COMPOUNDS OF Campylobacter spp. IN/FROM RETAIL POULTRY MEAT

Sonja Smole-Možina; Marija Kurinčić; Simona Uršić; Višnja Katalinić

Sonja Smole-Možina Biotechnical Faculty, Ljubljana, Slovenia
Marija Kurinčić Biotechnical Faculty, Ljubljana, Slovenia
Simona Uršić Institute of Public Health Celje
Višnja Katalinić Faculty of Chemistry and Technology, Split, Croatia

Keywords: food safety, poultry meat, Campylobacter, antibiotic resistance, disinfectant resistance, phenolic extracts

Abstract

The increasing antimicrobial resistance rates of microorganisms is an urgent world-wide problem, especially microbial multidrug resistance phenotypes, dispersed also among food-related bacteria. A case study could be the resistance of campylobacters, usually transmitted in the food chain by contaminated poultry meat. We tested the resistance of Campylobacter chicken meat isolates against a) selected antibiotics used in human and veterinary medicine - erythromycin, ciproﬂ oxacin, tetracycline, b) selected disinfectants used in food processing - benzalkonium chloride (BC), chlorhexidine diacetate (CHA), cetylpyridinium chloride (CPC) and c) alternative group of antimicrobial compounds - phenolic extracts from grape skins of different grape varieties. Campylobacter was isolated from retail chicken meat samples by standard (ISO 10272) isolation procedure. Beside classical phenotyping methods of species identiﬁ cation and resistotyping, polymerase chain reaction (PCR) and/or restriction fragment lenght polymorphisms of speciﬁ c amplicons (PCR-RFLPs) were used for species identiﬁ cation and determination of mutations in target genes. Antibiotic resistance phenotypes were studied by disk diffusion,
agar dilution and broth microdilution method with CellTiter-Blue reagent and automated ﬂ uorescence signal detection. The resistance to disinfectants and phenolic extracts was tested by broth microdilution method and expressed as minimal inhibitory concentrations (MICs). The involvement of efﬂ ux pumps in antibiotic and disinfectant resistance was assessed by measurements of MICs with and without addition of chemical efﬂ ux pump inhibitors, phenylalanine-arginine ß-naphthylamide (PAßN) and 1(1-naphthylmethyl)-piperazine (NMP) and by testing Campylobacter jejuni cmeB mutant strain. High prevalence
of antibiotic resistant strains was found among chicken meat isolates. Regarding ciproﬂoxacin, 66.5 % of tested strains were found resistant, including C. jejuni and C. coli strains, but resistance to erythromycin
was much more frequent among C. coli isolates (34.5 %, but 13.9 % in average among 158 tested isolates. Tetracycline resistance was relatively rare, but multidrug resistant strains were found. No signiﬁcant
difference in biocide susceptibility between antibiotic resistant and sensitive Campylobacter isolates was conﬁrmed. Finally, the results of initial screening of susceptibility of Campylobacter meat isolates against grape skin phenolic extracts tested are promising for further study of such antimicrobial compounds (or their mixtures) for potential use in assuring safety of poultry meat and other product.